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Rabbit Anti-Annexin V/Biotin Conjugated antibody (bs-0450R-Bio)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-0450R-Bio
英文名稱 Rabbit Anti-Annexin V/Biotin Conjugated antibody
中文名稱 生物素標(biāo)記的重組膜粘連蛋白5抗體
別    名 Anchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A5; ANX5; ANXA5; Calphobindin I; CBP I; CBP-I; Endonexin II; ENX 2; ENX2; Lipocortin V; PAP I; Placental anticoagulant protein I; PAP-I; Placental anticoagulant protein 4; PP 4; PP4; Thromboplastin inhibitor; VAC alpha; Vascular anticoagulant alpha; Vascular anticoagulant-alpha; ANXA5_HUMAN; Anchorin CII; Annexin-5; VAC-alpha.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 細(xì)胞凋亡  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, 
產(chǎn)品應(yīng)用 WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 36kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 recombind human Annexin V
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008].

Function:
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

Subunit:
Interacts with PDCD6.

Post-translational modifications:
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

DISEASE:
Defects in ANXA5 are associated with susceptibility to pregnancy loss, recurrent, type 3 (RPRGL3) [MIM:614391]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.

Similarity:
Belongs to the annexin family.
Contains 4 annexin repeats.

Database links:

Entrez Gene: 308 Human

Entrez Gene: 11747 Mouse

Entrez Gene: 25673 Rat

Omim: 131230 Human

SwissProt: P08758 Human

SwissProt: P48036 Mouse

SwissProt: P14668 Rat

Unigene: 480653 Human

Unigene: 1620 Mouse

Unigene: 3318 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Annexin-V是一種分子量為35~36KD的Ca2+依賴性磷脂結(jié)合蛋白,能與PS高親和力特異性結(jié)合。
將Annexin-V進(jìn)行熒光素(FITC、PE)或biotin標(biāo)記,以標(biāo)記了的Annexin-V作為熒光探針,利用流式細(xì)胞儀或熒光顯微鏡可檢測(cè)細(xì)胞凋亡的發(fā)生。
其意義:檢測(cè)早期凋亡的細(xì)胞,同時(shí)可區(qū)分活細(xì)胞和壞死細(xì)胞 磷脂酰絲氨酸(Phosphatidylserine,PS)正常位于細(xì)胞膜的內(nèi)側(cè),但在細(xì)胞凋亡的早期,PS可從細(xì)胞膜的內(nèi)側(cè)翻轉(zhuǎn)到細(xì)胞膜的表面,暴露在細(xì)胞外環(huán)境中。
碘化丙啶(propidine iodide,PI)是一種核酸染料,它不能透過完整的細(xì)胞膜,但在凋亡中晚期的細(xì)胞和死細(xì)胞,PI能夠透過細(xì)胞膜而使細(xì)胞核紅染。因此將Annexin-V與PI匹配使用,就可以將凋亡早晚期的細(xì)胞以及死細(xì)胞區(qū)分開來。
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