| 產(chǎn)品編號(hào) |
bs-0008R-PE-Cy3 |
| 英文名稱(chēng) |
Rabbit Anti-Nestin/PE-Cy3 Conjugated antibody
|
| 中文名稱(chēng) |
PE-Cy3標(biāo)記的巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體 |
| 別 名 |
ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin. |
| 規(guī)格價(jià)格 |
100ul/2980元
購(gòu)買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) |
100ul
|
| 研究領(lǐng)域 |
腫瘤 心血管 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 細(xì)胞類(lèi)型標(biāo)志物 腫瘤細(xì)胞生物標(biāo)志物 |
| 抗體來(lái)源 |
Rabbit |
| 克隆類(lèi)型 |
Polyclonal |
| 交叉反應(yīng) |
Mouse, Rat,
|
| 產(chǎn)品應(yīng)用 |
Flow-Cyt=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 |
209kDa |
| 性 狀 |
Lyophilized or Liquid |
| 濃 度 |
1mg/ml |
| 免 疫 原 |
KLH conjugated synthetic peptide derived from rat Nestin |
| 亞 型 |
IgG |
| 純化方法 |
affinity purified by Protein A |
| 儲(chǔ) 存 液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
cytoplasm
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
Database links:
Entrez Gene: 10763 Human
Entrez Gene: 18008 Mouse
Entrez Gene: 25491 Rat
Omim: 600915 Human
SwissProt: P48681 Human
SwissProt: Q6P5H2 Mouse
SwissProt: P21263 Rat
Unigene: 527971 Human
Unigene: 331129 Mouse
Unigene: 9701 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
神經(jīng)干細(xì)胞標(biāo)志物
在動(dòng)物體早期發(fā)育過(guò)程中，中樞神經(jīng)系統(tǒng)發(fā)育尤為重要 。Nestin是一種中等纖維蛋白，它在哺乳動(dòng)物神經(jīng)前體細(xì)胞中表達(dá)較高，目前已被廣泛用于神經(jīng)前體細(xì)胞的重要標(biāo)志分子。研究Nestin的表達(dá)調(diào)控規(guī)律，將有助于人們認(rèn)識(shí)神經(jīng)前體細(xì)胞的生物特性。此抗體還表達(dá)中樞神經(jīng)系統(tǒng)干細(xì)胞及神經(jīng)上皮細(xì)胞。
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