| 產品編號 | bs-7756R-Gold |
| 英文名稱 | Rabbit Anti-MyoGEF/Gold Conjugated antibody |
| 中文名稱 | 膠體金標記的肌球蛋白相互作用G蛋白交換因子抗體 |
| 別 名 | Myosin interacting guanine nucleotide exchange factor; PH domain containing family G member 6; Pleckstrin homology domain containing family G (with RhoGef domain) member 6; Pleckstrin homology domain-containing family G member 6; PLEKHG6；PKHG6_HUMAN。 |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul（10nm 15nm 35nm） |
| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞周期蛋白 轉錄調節(jié)因子 細胞分化 細胞骨架 細胞外基質 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Horse, Rabbit, ) |
| 產品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 89kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MyoGEF |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產品介紹 |
background: MyoGEF functions as a guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. It activates RHOG but not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving MyoGEF, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis. Function: Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis. Subunit: Interacts with MYH10. Interacts with ELMO1 and EZR (in an open conformation). Interacts with CSPP1. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cleavage furrow. Note=During mitosis, localizes to the spindle pole, central spindle and cleavage furrow. In epithelial cells, recruited to the apical membrane by EZR. Tissue Specificity: Highest expression in the placenta. Low levels in small intestine, lung, liver, kidney, thymus and heart. Similarity: Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Database links: UniProtKB/Swiss-Prot: Q3KR16.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
