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Rabbit Anti-Talin/FITC Conjugated antibody (bs-3619R-FITC)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-3619R-FITC
英文名稱 Rabbit Anti-Talin/FITC Conjugated antibody
中文名稱 FITC標(biāo)記的踝蛋白抗體
別    名 TLN 1; ILWEQ; Talin 1; TLN; TLN1; TLN1_HUMAN.  
規(guī)格價(jià)格 100ul/2980元 購買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  細(xì)胞粘附分子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, )
產(chǎn)品應(yīng)用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 280kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Talin
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.

Function:
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.

Subunit:
Binds with high affinity to VCL and with low affinity to integrins. Interacts with PTK2/FAK1 (By similarity). Interacts with PIP5K1C and NRAP. Interacts with LAYN. Interacts with SYNM.

Subcellular Location:
Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Note=Colocalizes with LAYN at the membrane ruffles.

Similarity:
FERM domain.
Contains 1 I/LWEQ domain.

Database links:

Entrez Gene: 395194 Chicken

Entrez Gene: 7094 Human

Entrez Gene: 21894 Mouse

Entrez Gene: 313494 Rat

Omim: 186745 Human

SwissProt: P54939 Chicken

SwissProt: Q9Y490 Human

SwissProt: P26039 Mouse

Unigene: 471014 Human

Unigene: 208601 Mouse

Unigene: 7715 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

TALIN-踝蛋白。是重要的細(xì)胞骨架肌動(dòng)蛋白結(jié)合蛋白,由兩個(gè)270kDa的亞單位組成的反向 平行的同源二聚體,與整合蛋白B尾部的結(jié)合誘導(dǎo)了整合蛋白胞外結(jié)構(gòu)域構(gòu)象的改變,起到了對(duì)整合蛋白的激活。
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