| 產(chǎn)品編號 | bs-2489R-Bio |
| 英文名稱 | Rabbit Anti-CD9/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的CD9蛋白抗體 |
| 別 名 | Tetraspanin 29; 5H9; 5H9 antigen; Antigen defined by monoclonal; 602 29; Antigen defined by monoclonal; 60229; BA 2; BA2; BTCC 1; BTCC1; CD 9; CD9; CD9 antigen; CD9 antigen p24; CD9 molecule; CD9_HUMAN; Cell growth inhibiting gene 2 protein; Cell growth-inhibiting gene 2 protein; DRAP 27; DRAP27; GIG 2; GIG2; Growth inhibiting gene 2 protein; Leukocyte antigen MIC3; MIC 3; MIC3; Motility related protein; Motility-related protein; MRP 1; MRP-1; MRP1; p24; P24; p24 antigen; Tetraspanin 29; Tetraspanin-29; Tetraspanin29; Tspan 29; Tspan-29; Tspan29. |
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Journal
PMID
IF
Application
[IF=14.976] Junli Zhang. et al. Localized fluorescent imaging of multiple proteins on individual extracellular vesicles using rolling circle amplification for cancer diagnosis. J Extracell Vesicles. 2020 Nov;10(1):e12025
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| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 發(fā)育生物學(xué) 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD9 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve. Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Subunit: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed by a variety of hematopoietic and epithelial cells. Post-translational modifications: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. Similarity: Belongs to the tetraspanin (TM4SF) family. Database links: Entrez Gene: 928 Human Entrez Gene: 12527 Mouse Omim: 143030 Human SwissProt: P21926 Human SwissProt: P40240 Mouse Unigene: 114286 Human Unigene: 210676 Mouse Unigene: 404614 Mouse Unigene: 2091 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD9是一種普遍存在于細胞膜表面屬于四跨膜超蛋白家族的細胞表面糖蛋白, 參與質(zhì)膜的融合過程，在細胞膜生物學(xué)中發(fā)揮重要作用。 近年來經(jīng)研究發(fā)現(xiàn)，CD9與多種腫瘤轉(zhuǎn)移有關(guān)，故稱腫瘤轉(zhuǎn)移抑制基因亦稱抗腫瘤轉(zhuǎn)移基因。是一個新的腫瘤轉(zhuǎn)移抑制基因膜運動相關(guān)蛋白-1(CD9/MRP-1)，其分子功能還有待于進一步研究。CD9主要在早B細胞、活化T淋巴細胞、嗜酸性粒細胞、嗜堿性粒細胞和血小板表達。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
