| 產(chǎn)品編號 | bs-1840R-AP |
| 英文名稱 | Rabbit Anti-Lamin B/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標(biāo)記的核纖層蛋白B抗體(細(xì)胞核膜標(biāo)志物) |
| 別 名 | lamin B1; LMB1; LMN; LMN2; LMNB 1; LMNB; LMNB1; MGC111419; LMNB1_HUMAN; Lamin-B1. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 細(xì)胞類型標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Rat, (predicted: Mouse, Pig, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 64kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Lamin B (401-480aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]. Function: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Subunit: Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Subcellular Location: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side. Post-translational modifications: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. DISEASE: Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. Similarity: Belongs to the intermediate filament family. Database links: Entrez Gene: 396223 Chicken Entrez Gene: 4001 Human Entrez Gene: 16906 Mouse Omim: 150340 Human SwissProt: P14731 Chicken SwissProt: P20700 Human SwissProt: P14733 Mouse Unigene: 89497 Human Unigene: 4105 Mouse Unigene: 11362 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 核膜標(biāo)志物（Nuclear Envelope Marker） 核纖層蛋白(Lamin) 是緊貼核內(nèi)膜的一層厚度為20～50nm的纖維蛋白層或纖維網(wǎng)絡(luò)。核纖層與細(xì)胞質(zhì)骨架、核骨架連成一個整體，一般認(rèn)為核纖層將核被膜和染色質(zhì)提供了結(jié)構(gòu)支架。有學(xué)者研究認(rèn)為：lamin蛋白與細(xì)胞凋亡及衰老有關(guān)聯(lián)，它包括：核纖層蛋白A、核纖層蛋白B、核纖層蛋白C幾個不同亞型的蛋白。 |
