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Rabbit Anti-SLC10A2  antibody (bs-41358R)  
~~~促銷(xiāo)代碼KT202411~~~
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產(chǎn)品編號(hào) bs-41358R
英文名稱(chēng) Rabbit Anti-SLC10A2  antibody
中文名稱(chēng) 頂膜鈉依賴(lài)性膽鹽轉(zhuǎn)運(yùn)體蛋白抗體
別    名 SLC10A2; Apical sodium dependent bile acid transporter; Apical sodium- dependent bile acid transporter; Ileal sodium dependent bile acid transporter; Ileal sodium-dependent bile acid transporter; Ileal sodium/bile acid cotransporter; ISBT; Na+ bile acid cotransporter; Na+ dependent ileal bile acid transporter; NTCP2; Sodium/taurocholate cotransporting polypeptide, ileal; solute carrier family 10 (sodium/bile acid cotransporter family); Solute carrier family 10 member 2.  
研究領(lǐng)域 腫瘤  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  通道蛋白  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 38kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant mouse SLC10A2 protein: 306-348/348 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 SLC10A2 plays a critical role in reabsorption of bile acids from the the small intestine lumen. Passive flow of sodium ions down their concentration gradient is coupled to bile acid movement, resulting in an increase in the concentration of bile acids in the interior of the cell. This action conserves the body's pool of re-circulating bile acid. SLC10A2 also plays a key role in cholesterol metabolism as cholesterol is the precursor molecule in bile acid synthesis mediated by CYP7A and FXR.

SWISS:
Q12908

Gene ID:
6555

Database links:

Entrez Gene: 6555 Human

Entrez Gene: 20494 Mouse

Entrez Gene: 29500 Rat

Omim: 601295 Human

SwissProt: Q12908 Human

SwissProt: P70172 Mouse

SwissProt: Q62633 Rat

Unigene: 194783 Human

Unigene: 3500 Mouse

Unigene: 85891 Rat




產(chǎn)品圖片
Sample: Lane 1: Human LOVO cell lysates Lane 2: Human SW480 cell lysates Lane 3: Human 293T cell lysates Primary: Anti-SLC10A2 (bs-41358R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 46 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC10A2) Polyclonal Antibody, Unconjugated (bs-41358R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Measured by its binding ability in a indirect ELISA. Immobilized Mouse SLC10A2 protein, His Tag (Cat. bs-41358P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Mouse SLC10A2 Antibody, the EC50 is 179.9 ng/mL.
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