| 產(chǎn)品編號 | bsm-54186R |
| 英文名稱 | HDAC9 Recombinant Rabbit mAb |
| 中文名稱 | 組蛋白去乙?；?重組兔單抗 |
| 別 名 | HD 7; HD 7B; HD 9; HD7; HD7B; HD9; HDAC 7; HDAC 7B; HDAC 9; HDAC 9B; HDAC 9FL; HDAC; HDAC7; HDAC7B; HDAC9B; HDAC9FL; HDRP; Histone deacetylase 4/5 related protein; Histone deacetylase 7; Histone deacetylase 7B; Histone deacetylase 9; Histone deacetylase 9A; Histone deacetylase related protein; KIAA0744; MEF2 interacting transcription repressor MITR; MEF2 interacting transcription repressor protein; MITR. DKFZp779K1053; HDAC9_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 111 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human HDAC9: 1-38 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HDAC9 (Histone Deacetylase 9) catalyses the deacetylation of lysine residues on the core histones (H2A, H2B, H3 and H4). It belongs to the Class IIa HDAC family, which are key regulators of several developmental and differentiation processes such as cardiac growth. HDAC9 represses MEF2 (myocyte enhancer factor 2)-dependent transcription, playing an important role in cardiac hypertrophy. The HDAC9 isoform MITR/HDRP lacks active site residues and is therefore catalytically inactive. It represses MEF2-dependent transcription by interacting with HDAC1 and/or HDAC3. MITR/HDRP is thought to play a role in skeletal myogenesis and in heart development. It also protects neurons from apoptosis by inhibiting c-Jun phosphorylation by MAPK10 and by repressing c-Jun transcription through recruitment of HDAC1 to the c-Jun promoter. Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter. Subunit: Homodimer. Interacts with CTBP1. The phosphorylated form interacts with 14-3-3. Interacts with HDAC1 and HDAC3, and probably with HDAC4 and HDAC5. Interacts with MEF2, MAPK10, ETV6, NCOR1 and BCL6. Subcellular Location: Nucleus. Tissue Specificity: Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level). Post-translational modifications: Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation. Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Sumoylated. DISEASE: Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein. Similarity: Belongs to the histone deacetylase family. HD type 2 subfamily. SWISS: Q9UKV0 Gene ID: 9734 Database links: Entrez Gene: 9734 Human Entrez Gene: 79221 Mouse Omim: 606543 Human SwissProt: Q9UKV0 Human SwissProt: Q99N13 Mouse Unigene: 196054 Human Unigene: 310551 Mouse Unigene: 483009 Mouse |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with HDAC9 monoclonal antibody, unconjugated (bsm-54186R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Raji (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HDAC9) monoclonal Antibody, unconjugated (bsm-54186R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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