| 產(chǎn)品編號 | bsm-60005R |
| 英文名稱 | Mesothelin Recombinant Rabbit mAb |
| 中文名稱 | 間皮素重組兔單抗 |
| 別 名 | MSLN; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 腫瘤細(xì)胞生物標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 1A14 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:1000,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Mesothelin: 550-570/630 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Mesothelin(MSLN)may play a role in cell adhesion and cell shape dynamics; human homolog highly expressed in renal cell carcinoma. malignant tumors, such as a skin lump, ovary cancer and the pancreas cancer...etc..Mainly used for the research of the malignant tumor. Function: Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Subunit: Interacts with MUC16. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Megakaryocyte-potentiating factor: Secreted. Isoform 3: Secreted. Tissue Specificity: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). Post-translational modifications: Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. Similarity: Belongs to the mesothelin family. SWISS: Q13421 Gene ID: 10232 Database links: Entrez Gene: 10232 Human Omim: 601051 Human SwissProt: Q13421 Human Unigene: 408488 Human |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at
room temperature
Lysate: HeLa
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 69 kDa
Observed MW: 40, 69 kDa
Paraformaldehyde-fixed, paraffin embedded (Human skin tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mesothelin) Monoclonal Antibody, Unconjugated (bsm-60005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mesothelin) Monoclonal Antibody, Unconjugated (bsm-60005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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