| 產(chǎn)品編號 | bsm-52574R |
| 英文名稱 | Tyrosine Hydroxylase Recombinant Rabbit mAb |
| 中文名稱 | 酪氨酸羥化酶兔單克隆抗體 |
| 別 名 | DYT14; DYT5b; ple; Protein Pale; c; The; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyk2; TY3H_HUMAN; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 8B6 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Tyrosine hydroxylase: 500-528 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008] Function: Plays an important role in the physiology of adrenergic neurons. Tissue Specificity: Mainly expressed in the brain and adrenal glands. DISEASE: Defects in TH are the cause of Segawa syndrome autosomal recessive (ARSEGS) [MIM:605407]. A form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA. Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. Similarity: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. SWISS: P07101 Gene ID: 7054 Database links: Entrez Gene: 7054 Human Entrez Gene: 21823 Mouse Omim: 191290 Human SwissProt: P07101 Human SwissProt: P24529 Mouse Unigene: 435609 Human Unigene: 1292 Mouse Unigene: 11082 Rat 神經(jīng)細(xì)胞標(biāo)志物 酪氨酸羥化酶（TH）是兒茶酚胺類神經(jīng)遞質(zhì)即多巴胺、去甲腎上腺素、腎上腺素生物合成過程所需的限速酶，它以四氫生物喋呤啶(BH4)為輔酶，催化酪氨酸的羥化而生成多巴(DOPA)。 已知在患帕金森病(Parkinson disease,PD)時，腦內(nèi)多巴胺(dopamine,DA)的減少與此酶活性低下有關(guān)。因此對PD模型動物來說，若將TH基因植入腦內(nèi)，便可以提高腦內(nèi)DA水平而達(dá)到基因治療目的。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Tyrosine Hydroxylase monoclonal antibody, unconjugated (bsm-52574R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tyrosine Hydroxylase Monoclonal Antibody, Unconjugated(bsm-52574R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tyrosine Hydroxylase Monoclonal Antibody, Unconjugated(bsm-52574R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tyrosine Hydroxylase Monoclonal Antibody, Unconjugated(bsm-52574R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tyrosine Hydroxylase Monoclonal Antibody, Unconjugated(bsm-52574R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tyrosine Hydroxylase Monoclonal Antibody, Unconjugated(bsm-52574R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tyrosine Hydroxylase Monoclonal Antibody, Unconjugated(bsm-52574R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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