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EMT Research Antibody Assembly kit (E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1) (Abk003)  
訂購熱線:400-901-9800
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25ul×5/3000.00元
50ul×5/5600.00元
100ul×5/8500.00元
大包裝/詢價(jià)

產(chǎn)品編號(hào) Abk003
英文名稱 EMT Research Antibody Assembly kit (E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1)
中文名稱 EMT標(biāo)志物抗體套裝
別    名 EMT Research Antibody Assembly kit(E-Cadherin; N-Cadherin; Vimentin; Occludin; ZO-1)  上皮-間質(zhì)轉(zhuǎn)換(EMT)標(biāo)志物抗體套裝(E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1); EMT標(biāo)志物抗體套裝
研究領(lǐng)域 腫瘤  細(xì)胞生物  細(xì)胞粘附分子  細(xì)胞表面分子  細(xì)胞外基質(zhì)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) For research use only. Not intended for diagnostic or therapeutic use.
PubMed PubMed
產(chǎn)品介紹 Epithelial-mesenchymal transition (EMT) is a biological process that allows epithelial cell to assume mesenchymal phenotype. EMT and its reverse process, mesenchymal-epithelial transition (MET), are originally defined in the context of developmental stages and re-engaged in adults during wound healing, tissue regeneration, organ fibrosis, and cancer progression. Epithelial cells normally interacts with basement membrane via its basal surface. During EMT, they lose their cell polarity and cell-cell adhesion, and gain mesenchymal cell phenotype, including enhanced migratory capacity, invasiveness, elevated resistance to apoptosis, stem-like features, and increased production of ECM components. Accordingly, there is reprogrammed gene expression with EMT. Especially, some cytoskeleton proteins (such as cadherin family members), tight-junction proteins (such as Occludin and ZO-1) and intermediate filament of mesenchymal origin (such as Vimentin) undergo significant changes and serve as best EMT markers to evaluate EMT.

EMT Research Antibody Assembly kit is one economical package of selected antibodies for most representative EMT markers, including E-Cadherin, N-Cadherin, Vimentin, Occludin, and ZO-1. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet.

EMT Research Antibody Assembly kit
Rabbit Anti-E cadherin antibody (bs-1519R)
Rabbit Anti-N-cadherin antibody (bs-1172R)
Rabbit Anti-Vimentin antibody (bs-0756R)
Rabbit Anti-Occludin antibody (bs-10011R)
Rabbit Anti-ZO-1 antibody (bs-1329R)


Database links:

EMT Research Antibody Assembly kit

Rabbit Anti-E cadherin antibody (bs-1519R)

Rabbit Anti-N-cadherin antibody (bs-1172R)

Rabbit Anti-Vimentin antibody (bs-0756R)

Rabbit Anti-Occludin antibody (bs-10011R)

Rabbit Anti-ZO-1 antibody (bs-1329R)




上皮-間質(zhì)轉(zhuǎn)換(EMT)是一個(gè)使上皮細(xì)胞呈現(xiàn)間質(zhì)細(xì)胞表型的生物學(xué)轉(zhuǎn)變過程,EMT及其反向過程,間質(zhì)-上皮轉(zhuǎn)換(MET),最初定義在早期發(fā)育階段,并在成年期重新參與到傷口愈合、組織再生、器官纖維化和癌癥進(jìn)展等多種病理過程中。上皮細(xì)胞通常與基底膜緊密結(jié)合,在EMT過程中,它們失去細(xì)胞極性和細(xì)胞粘附并獲得間質(zhì)細(xì)胞表型,包括遷移能力、侵襲性、對(duì)凋亡的抗性增強(qiáng)、干細(xì)胞樣特征和ECM組分的增加。與此相應(yīng)很多基因表達(dá)也發(fā)生了重新編程,尤其是一些細(xì)胞骨架蛋白(如鈣粘素家族成員)、緊密連接蛋白(如Occludin和ZO-1)和間質(zhì)特有中間纖維(如Vimentin)發(fā)生了顯著變化。因此,這些蛋白可作為評(píng)價(jià)EMT的最佳標(biāo)志。 EMT標(biāo)志物抗體套裝極具性價(jià)比,包含了最具代表性的EMT標(biāo)記物,包括E-cadherin、N-cadherin、Vimentin、Occludin和ZO-1。這些抗體在多種應(yīng)用和物種中進(jìn)行了測(cè)試。有關(guān)抗體具體使用信息,請(qǐng)參閱各個(gè)抗體產(chǎn)品數(shù)據(jù)表。
產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 monoclonal antibody, unconjugated (bsm-41327M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Vimentin polyclonal antibody, unconjugated (bs-8533R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with N Cadherin monoclonal antibody, unconjugated (bsm-52389R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with E cadherin monoclonal antibody, unconjugated (bsm-60814R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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