| 產(chǎn)品編號 | bs-22203R |
| 英文名稱 | Artemin Rabbit pAb |
| 中文名稱 | Artemin抗體 |
| 別 名 | ARTN_HUMAN; ARTN; Enovin; EVN; Neublastin; ART; NBN; |
| 研究領(lǐng)域 | 發(fā)育生物學(xué) 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12/19 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Artemin: 141-224/224 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein signals through the RET receptor and GFR alpha 3 coreceptor, and supports the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. This protein has also been shown to promote tumor growth, metastasis, and drug resistance in mammary carcinoma. [provided by RefSeq, Aug 2016] Function: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity). Subunit : Homodimer; disulfide-linked. Binds to RET. Subcellular Location: Secreted. Tissue Specificity: Cochlea. Expressed at higher level in sesorineural epithelium than in the modiolus region or substantia nigra. Similarity: Belongs to the TGF-beta family. GDNF subfamily. SWISS: Q9Z0L2 Gene ID: 11876 Database links: Entrez Gene: 9048 Human Entrez Gene: 11876 Mouse Omim: 603886 Human SwissProt: Q5T4W7 Human SwissProt: Q9Z0L2 Mouse Unigene: 632404 Human Unigene: 56897 Mouse Unigene: 154711 Rat 神經(jīng)生物學(xué)相關(guān)蛋白（Neurobiology） Artemin 是GDNF 家族的一個新成員，已被證實它是從前的孤兒受體GFRalpha3-RET的配體。 Artemin是感覺和交感神經(jīng)元培養(yǎng)的一個存活因子，它的表達形式也影響體內(nèi)的這些神經(jīng)元。Artemin 還能激活GFRalpha1-RET 復(fù)合物并支持培養(yǎng)中腦多巴胺神經(jīng)元存活 |
| 產(chǎn)品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Artemin (bs-22203R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/19 kD
Observed band size: 19 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (bs-22203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (bs-22203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
