| 產(chǎn)品編號 | bsm-51331M |
| 英文名稱 | Mouse Anti-RAB27B antibody |
| 中文名稱 | RAB27B單克隆抗體 |
| 別 名 | C25KG; Rab27b; RAS associated protein RAB27B; Ras related protein Rab 27b; Ras related protein Rab27b; Ras-related protein Rab-27B; RB27B_HUMAN; Small GTP binding protein rab27b; RAB27B, Member RAS Oncogene Family; |
| 研究領(lǐng)域 | 腫瘤 細胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 合成與降解 轉(zhuǎn)運蛋白 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 4G7 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human RAB27B |
| 亞 型 | IgG1,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RAB27B (RAB27B, Member RAS Oncogene Family) is a Protein Coding gene. Among its related pathways are Vesicle-mediated transport and Sertoli-Sertoli Cell Junction Dynamics. GO annotations related to this gene include GTP binding and protein domain specific binding. An important paralog of this gene is RAB27A. Function: May be involved in targeting uroplakins to urothelial apical membranes. Subunit: Interacts with SYTL2, SYTL4, MYRIP and MLPH. Interacts with RPH3A and RPH3A (By similarity). Subcellular Location: Membrane; Lipid-anchor Tissue Specificity: Expressed primarily in testis. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: O00194 Gene ID: 5874 Database links: Entrez Gene: 5874 Human Entrez Gene: 80718 Mouse Omim: 603869 Human SwissProt: O00194 Human SwissProt: Q99P58 Mouse Unigene: 25318 Human |
| 產(chǎn)品圖片 |
Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-RAB27B (bsm-51331M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 25 kD
Sample:
Bladder (Mouse) Lysate at 40 ug
Primary: Anti-RAB27B (bsm-51331M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- RAB27B (bsm-51331M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 24 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
