| 產(chǎn)品編號 | bsm-33019M |
| 英文名稱 | GFP Mouse mAb |
| 中文名稱 | 綠色熒光蛋白單克隆抗體 |
| 別 名 | Green fluorescence protein; 綠色熒光蛋白 GFP; 綠色熒光蛋白 (GFP); |
| 產(chǎn)品類型 | 標簽抗體 |
| 研究領域 | 細胞生物 海洋生物 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 4G6 |
| 交叉反應 | Recombinant protein |
| 產(chǎn)品應用 | WB=1:50000-500000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant Green Fluorescence Protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Subunit: Monomer. Tissue Specificity: Photocytes. Post-translational modifications: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Similarity: Belongs to the GFP family. Database links:
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| 產(chǎn)品圖片 |
Sample:
GFP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Primary:
Anti-GFP-Tag (bsm-33019M) at 1/1000000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 30 kD
Transformed (GFP or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bsm-33019M (Anti-GFP) at dilution of 1:1,000,000 incubated at 4℃ overnight.
Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 2 ug
Primary: Anti-GST tag (bsm-33019M) at 1/5000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
