| 產(chǎn)品編號 | bs-4977R |
| 英文名稱 | ADAM12 Rabbit pAb |
| 中文名稱 | 去整合素樣金屬蛋白酶12 |
| 別 名 | MLTN; A disintegrin and metalloproteinase domain 12; A disintegrin and metalloproteinase domain 12; ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; Disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; MCMPMltna; Meltrin alpha; Meltrin-alpha; MLTN; MLTNA; OTTHUMP00000046766. |
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Specific References (1) | bs-4977R has been referenced in 1 publications.
[IF=1.718] Branco DC et al.HIF-1α, NOTCH1, ADAM12 and HB-EGF are overexpressed in mucoepidermoid carcinoma. (2018) Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. (18)31198-2 IHC ; Human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞粘附分子 細(xì)胞骨架 細(xì)胞外基質(zhì) 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 77/100 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAM12/MLTN: 251-350/909 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNF?(tumor necrosis factor ?, and modulation of the neurogenic function of Notch and Delta (1-3). ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail (1-3). ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone, and muscle, and catalyze proteolysis, adhesion, fusion, and intracellular signaling (3). ADAM 12 (Meltrin-a) is produced as 2 differentially spliced isoforms, a 718 amino acid secreted form (ADAM12S) and a 881 amino acid membrane-bound form (ADAM12L), and is involved in egg-sperm fusion (4-6). Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). Subunit: Interacts with alpha-actinin-2 and with syndecans (By similarity). Interacts with SH3PXD2A. Interacts with FST3. Interacts with GNB2L1/RACK1; the interaction is required for PKC-dependent translocation of ADAM12 to the cell membrane. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted (Potential). Isoform 4: Secreted (Potential). Tissue Specificity: Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas. Post-translational modifications: The precursor is cleaved by a furin endopeptidase (By similarity). Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. SWISS: O43184 Gene ID: 8038 Database links: Entrez Gene: 8038 Human Entrez Gene: 11489 Mouse Omim: 602714 Human SwissProt: O43184 Human SwissProt: Q61824 Mouse Unigene: 594537 Human Unigene: 439714 Mouse |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ADAM12 polyclonal antibody, unconjugated (bs-4977R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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