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Rabbit Anti-ICAM4  antibody (bs-7637R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-7637R
英文名稱 Rabbit Anti-ICAM4  antibody
中文名稱 細(xì)胞間粘附分子4抗體
別    名 CD242; CD242 antigen; ICAM-4; Icam4; ICAM4_HUMAN; Intercellular adhesion molecule 4; Landsteiner Wiener blood group glycoprotein; Landsteiner-Wiener blood group glycoprotein; LW; LW blood group protein.  
Specific References  (1)     |     bs-7637R has been referenced in 1 publications.
[IF=3.23] Polin, Helene, et al. "Compound heterozygosity of two novel RHAG alleles leads to a considerable disruption of the Rh complex." Transfusion 56.4 (2016): 950-955.  FCM ;  Human.  
研究領(lǐng)域 心血管  細(xì)胞生物  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  細(xì)胞粘附分子  細(xì)胞骨架  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 27kDa
細(xì)胞定位 細(xì)胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ICAM4: 85-180/271 <Extracellular>
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.

Function:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.

Subcellular Location:
Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Secreted.

Tissue Specificity:
Erythrocytes.

Post-translational modifications:
N- and O-glycosylated.

Similarity:
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
Q14773

Gene ID:
3386

Database links:

Entrez Gene: 3386 Human

Entrez Gene: 78369 Mouse

Entrez Gene: 298702 Rat

Omim: 614088 Human

SwissProt: Q14773 Human

SwissProt: Q9ERM2 Mouse

Unigene: 706750 Human

Unigene: 30220 Mouse

Unigene: 222410 Rat



產(chǎn)品圖片
Sample: placenta(Mouse) Lysate at 40 ug Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kD
Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kD
Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kD
Blank control:K562. Primary Antibody (green line): Rabbit Anti-ICAM4 antibody (bs-7637R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:K562. Primary Antibody (green line): Rabbit Anti-ICAM4 antibody (bs-7637R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-7637R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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