| 產(chǎn)品編號 | bs-6575R |
| 英文名稱 | S100A7 Rabbit pAb |
| 中文名稱 | S100鈣結(jié)合蛋白A7抗體 |
| 別 名 | HID 5; Protein S100 A7; Protein S100-A7; PSOR 1; PSOR1; Psoriasin 1; Psoriasin; Psoriasin1; S100 Calcium binding protein A7; S100 calcium-binding protein A7; S100A7; S100A7c; S10A7_HUMAN; S115A_MOUSE; S100A7A; S100A15. |
 |
Specific References (1) | bs-6575R has been referenced in 1 publications.
[IF=4.52] Hau, Carren S., et al. "Visfatin Enhances the Production of Cathelicidin Antimicrobial Peptide, Human ??-Defensin-2, Human ??-Defensin-3, and S100A7 in Human Keratinocytes and Their Orthologs in Murine Imiquimod-Induced Psoriatic Skin."he American Journal of Pathology (2013).. IHC-P ; Mouse.
|
| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 結(jié)合蛋白 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse S100A7: 41-108/108 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients. Subunit: Interacts with RANBP9. Subcellular Location: Cytoplasm. Secreted. Note=Secreted by a non-classical secretory pathway. Tissue Specificity: Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients. Similarity: Belongs to the S-101 family. Contains 2 EF-hand domains. Database links: Entrez Gene: 381493 Mouse SwissProt: Q6S5I3 Mouse |
| 產(chǎn)品圖片 |
Tissue/cell: rat skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-S100A7 Polyclonal Antibody, Unconjugated(bs-6575R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-S100A7 antibody (bs-6575R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
