| 產(chǎn)品編號(hào) | bs-5605R |
| 英文名稱(chēng) | phospho-TERT (Ser1125) Rabbit pAb |
| 中文名稱(chēng) | 磷酸化端粒酶逆轉(zhuǎn)錄酶抗體 |
| 別 名 | p-TERT(Ser1125); TERT(phospho Ser1125); TERT(phospho S1125); EST2; hEST2; TCS1; Telomerase associated protein 2; Telomerase Catalytic Subunit; Telomere Reverse Transcriptase; TERT; TP2; TRT. |
| 產(chǎn)品類(lèi)型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=0.2μg/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 124 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human TERT around the phosphorylation site of Ser1125: LP(p-S)DF |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Telomerase are large DNA-protein complexes with telomerase expression being the subject of recent research due to its link to cell immortalization. Recent evidence has shown that MYC upregulates the catalytic subunit of telomerase, TERT, and that TERT cooperates with HPV E7 in cell immortalization. Ever since the discovery that telomeres are short in cancer cells and telomerase is activated in immortal cells, telomerase has been associated with oncogenes. During the past year, major advances have been made in understanding the link between telomerase expression and cell immortality. Studies of yeast telomeres have revealed an unexpected role for the non-homologous end-joining machinery in telomere maintenance and have provided the first definitive evidence that telomeres play a critical role in meiosis. Identification of new telomere proteins has led to a better understanding of vertebrate telomere structure and function. SWISS: O14746 Gene ID: 7015 Database links: Entrez Gene: 7015 Human Entrez Gene: 21752 Mouse SwissProt: O14746 Human SwissProt: O70372 Mouse ????端粒酶逆轉(zhuǎn)錄酶hTERT是構(gòu)成端粒酶的組分之一,是端粒酶活性的必需和限速成分,其水平?jīng)Q定細(xì)胞端粒酶的活性.抑制hTERT可降低端粒酶的活性,從而抑制瘤細(xì)胞生長(zhǎng).目前對(duì)hTERT的研究已成為端粒酶研究的熱點(diǎn)問(wèn)題,已發(fā)現(xiàn)TERT蛋白表達(dá)在腫瘤診斷中有重要意義,并制備了hTERT抗體及應(yīng)用核酶技術(shù)等來(lái)抑制hTERT蛋白的表達(dá),抑制端粒酶活性,從而抑制腫瘤的生長(zhǎng). ????端粒反轉(zhuǎn)錄酶又稱(chēng)端粒酶催化亞單位 （hTRT；Telomerase catalytic subunit；HEST2；Telomerase-associated protein 2；TP2；Telomerase reverse transcriptase；telomerase catalytic subunit）是細(xì)胞永生化及惡性腫瘤發(fā)生過(guò)程中的端粒酶活化的主要限速步驟。hTRT基因表達(dá)可以反映端粒酶活性，與端粒酶活性具有平行關(guān)系. |
| 產(chǎn)品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-TERT (Ser1125) (bs-5605R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-TERT (Ser1125)) polyclonal Antibody, Unconjugated (bs-5605R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (phospho-TERT (Ser1125)) polyclonal Antibody, Unconjugated (bs-5605R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-phospho-TERT(Ser1125) antibody (bs-5605R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-phospho-TERT (Ser1125) antibody (bs-5605R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-phospho-TERT (Ser1125) antibody (bs-5605R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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