| 產(chǎn)品編號 | bs-2517R |
| 英文名稱 | Rabbit Anti-CD72 antibody |
| 中文名稱 | CD72蛋白抗體 |
| 別 名 | B-cell differentiation antigen CD72; CD72b; B cell differentiation antigen CD72; CD72 antigen; CD72 molecule; CD72c; Ly-19; Ly-32; Ly-m19; Lyb-2; Lyb2; LYB2, mouse, homolog of; Lymphocyte antigen 32; MGC108873; CD72_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 干細胞 細胞表面分子 糖蛋白 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD72: 4-110/359 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface molecules such as the asialoglycoprotein receptors, CD23 and the Kupffer cell receptor. The function of CD72 is unknown but the exposure of B cells to CD72 antibodies activates a variety of signaling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages. Function: Plays a role in B-cell proliferation and differentiation. Subunit: Homodimer; disulfide-linked. Associates with CD5. Interacts (tyrosine phosphorylated) with PTPN6/SHP-1 (By similarity). Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells. Post-translational modifications: Phosphorylated upon engagement of the B-cell receptor, probably by LYN or SYK. Phosphorylation at Tyr-7 is important for interaction with PTPN6/SHP-1 (By similarity). Similarity: Contains 1 C-type lectin domain. SWISS: P21854 Gene ID: 971 Database links: Entrez Gene: 971 Human Omim: 107272 Human SwissProt: P21854 Human Unigene: 116481 Human CD72是一個重要的B細胞特異性受體。屬于II型膜內(nèi)糖蛋白成員中的細胞表面分子非唾液酸糖蛋白受體，雖然其分子功能目前還不清楚，但目前發(fā)現(xiàn)CD72的多態(tài)性與多種腫瘤有關(guān)。 |
| 產(chǎn)品圖片 |
Blank control(blue):Raji cells (fixed with 2% paraformaldehyde (10 min)) . Primary Antibody:Rabbit Anti- CD72 antibody(bs-2517R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
