| 產(chǎn)品編號(hào) | bs-2489R |
| 英文名稱(chēng) | Rabbit Anti-CD9 antibody |
| 中文名稱(chēng) | CD9蛋白抗體 |
| 別 名 | CD9 antigen; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29; CD9_HUMAN. |
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Specific References (14) | bs-2489R has been referenced in 14 publications.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 發(fā)育生物學(xué) 細(xì)胞表面分子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24kDa |
| 檢測(cè)分子量 | 24 |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD9: 101-200/228 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve. Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Subunit: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed by a variety of hematopoietic and epithelial cells. Post-translational modifications: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. Similarity: Belongs to the tetraspanin (TM4SF) family. SWISS: P21926 Gene ID: 928 Database links: Entrez Gene: 928 Human Entrez Gene: 12527 Mouse SwissProt: P21926 Human SwissProt: P40240 Mouse CD9是一種普遍存在于細(xì)胞膜表面屬于四跨膜超蛋白家族的細(xì)胞表面糖蛋白, 參與質(zhì)膜的融合過(guò)程，在細(xì)胞膜生物學(xué)中發(fā)揮重要作用。 近年來(lái)經(jīng)研究發(fā)現(xiàn)，CD9與多種腫瘤轉(zhuǎn)移有關(guān)，故稱(chēng)腫瘤轉(zhuǎn)移抑制基因亦稱(chēng)抗腫瘤轉(zhuǎn)移基因。是一個(gè)新的腫瘤轉(zhuǎn)移抑制基因膜運(yùn)動(dòng)相關(guān)蛋白-1(CD9/MRP-1)，其分子功能還有待于進(jìn)一步研究。CD9主要在早B細(xì)胞、活化T淋巴細(xì)胞、嗜酸性粒細(xì)胞、嗜堿性粒細(xì)胞和血小板表達(dá)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Placenta (Mouse) Lysate at 40 ug
Lane 3: Testis (Rat) Lysate at 40 ug
Primary:
Anti-CD9 (bs-2489R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 24 kD
Blank control: Mouse spleen cells(blue). Primary Antibody:Rabbit Anti-CD9 antibody(bs-2489R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: RSC96 cells (blue). Primary Antibody:Rabbit Anti-CD9 antibody(bs-2489R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: Raji(blue).
Primary Antibody: Rabbit Anti-CD9 antibody(bs-2489R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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