| 產(chǎn)品編號 | bs-1777R |
| 英文名稱 | CCKBR Rabbit pAb |
| 中文名稱 | 促胃泌素受體抗體 |
| 別 名 | Gastrin receptor; GASR; CCK B; CCK B receptor; CCK BR; CCK-B receptor; CCK-BR; CCK2 R; CCK2 receptor; CCK2-R; CCKB; CCKB receptor; CCKBR; CCKR Type B; CCKRB; Cholecystokinin 2 receptor; Cholecystokinin B receptor; Cholecystokinin-2 receptor; Cholecystokinin2 receptor; CholecystokininB receptor; GASR; GASR_HUMAN; Gastrin receptor; Gastrin/cholecystokinin type B receptor; Gastrin\cholecystokinin brain receptor. |
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Specific References (4) | bs-1777R has been referenced in 4 publications.
[IF=5.383] Ting Xiang. et al. Spinal CCK1 receptors contribute to somatic pain hypersensitivity induced by malocclusion via a reciprocal neuron-glial signaling cascade. J PAIN. 2022 Jun;: WB ; Rat.
[IF=4.432] Lu-Lu Duan. et al. Spinal CCK contributes to somatic hyperalgesia induced by orofacial inflammation combined with stress in adult female rats. Eur J Pharmacol. 2021 Dec;913:174619 WB ; Rat.
[IF=4.26] Mohammad, Shahid, et al. "Functional compensation between cholecystokinin-1 and-2 receptors in murine paraventricular nucleus neurons." Journal of Biological Chemistry 287.47 (2012): 39391-39401. IHC ; Mouse.
[IF=4.26] Mohammad et al. Functional compensation between cholecystokinin-1 and -2 receptors in murine paraventricular nucleus neurons. (2012) J.Biol.Che. 287:39391-401 IHC ; Mouse.
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| 研究領(lǐng)域 | 免疫學(xué) 神經(jīng)生物學(xué) 生長因子和激素 內(nèi)分泌病 G蛋白偶聯(lián)受體 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Gastrin receptor: 301-400/447 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism. Function: Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC-28 cell lines, not expressed in GLC-19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P32239 Gene ID: 887 Database links: Entrez Gene: 887 Human Entrez Gene: 12426 Mouse Omim: 118445 Human SwissProt: P32239 Human SwissProt: P56481 Mouse Unigene: 203 Human Unigene: 44513 Mouse Unigene: 90997 Rat |
| 產(chǎn)品圖片 |
Sample: Stomach(Mouse) Lysate at 40 ug
Primary: Anti- CCKBR? (bs-1777R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 53 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- CCKBR (bs-1777R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCKBR) Polyclonal Antibody, Unconjugated (bs-1777R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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