| 產品編號 | bs-1557G |
| 英文名稱 | HBsAg Goat pAb |
| 中文名稱 | 山羊抗人乙肝表面抗原抗體（包被） |
| 別 名 | L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen; S; HBSAG_HBVC5. |
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Specific References (16) | bs-1557G has been referenced in 16 publications.
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| 研究領域 | 微生物學 細菌及病毒 |
| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,HBV (predicted: Bee,Hepatitis B virus) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant HBsAg protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr. Function: The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. Subunit: Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion. Subcellular Location: Virion membrane. Post-translational modifications: Isoform M is N-terminaly acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region. Myristoylated. Similarity: Belongs to the orthohepadnavirus major surface antigen family. Database links: Entrez Gene: 2828293 Hepatitis B virus Entrez Gene: 944569 Hepatitis B virus SwissProt: P03138 Hepatitis B virus SwissProt: P03140 Hepatitis B virus SwissProt: P03141 Hepatitis B virus SwissProt: Q81157 Hepatitis B virus SwissProt: Q81158 Hepatitis B virus SwissProt: Q81159 Hepatitis B virus SwissProt: Q81160 Hepatitis B virus |
| 產品圖片 |
Sample:
Lane 1: Recombinant HBsAg protein(bs-41420P)
Primary: Anti-HBsAg (bs-1557G) at 1/1000 dilution
Secondary: IRDye800CW Donkey anti-Goat IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 20 kDa
Paraformaldehyde-fixed, paraffin embedded (Hepatitis B virus infected human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HBsAg) Polyclonal Antibody, Unconjugated (bs-1557G) at 1:200 overnight at 4°C, followed by A594 conjugated Rabbit Anti-Goat IgG H&L secondary antibody (bs-0294R-A594) incubation and staining.
Measured by its binding ability in a indirect ELISA. Immobilized recombinant HBsAg protein (Cat. bs-41420P) at 2 μg/mL (100 μL/well) can bind ?Goat Anti-Human HBsAg Antibody, the EC50 is 29.14 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
