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[IF=7.94] Maolin Zhang. et al. Rapid and efficient generation of cartilage pellets from mouse induced pluripotent stem cells by transcriptional activation of BMP-4 with shaking culture:. J TISSUE ENG. 2022;():  IF ;  Mouse.  

PubMed:35923173

[IF=6.064] Xinchao Miao. et al. Epiprofin Transcriptional Activation Promotes Ameloblast Induction From Mouse Induced Pluripotent Stem Cells via the BMP-Smad Signaling Axis. FRONT BIOENG BIOTECH. 2022 Jun 21;10:890882  IF ;  Mouse.  

PubMed:35800329

[IF=2.766] Kim et al. Klotho and S100A8/A9 as Discriminative Markers between Pre-Renal and Intrinsic Acute Kidney Injury. (2016) PLoS.One. 11:e0147255  IF ;  Rat.  

PubMed:26799323

[IF=1.888] C Geng et al. A simple fabricated microfluidic chip for urine sample-based bladder cancer detection.(2018).J MICROMECH MICROENG.  microfluidic chip ;  human.  

PubMed:10.1088/1361-6439/aae016

[IF=1.31] Cong, Shan, Guifang Cao, and Dongjun Liu. "Effects of different feeder layers on culture of bovine embryonic stem cell-like cells in vitro." Cytotechnology (2014): 1-11.  Bovine.  

PubMed:24807816

[IF=0] Zschemisch, Nils-Holger, et al. "Immortalized tumor derived rat fibroblasts as feeder cells facilitate the cultivation of male embryonic stem cells from the rat strain WKY/Ztm." SpringerPlus 3.1 (2014): 588.  Rat.  

PubMed:25332888

Sample:A549 Cell (Human) Lysate at 40 ug Primary: Anti-FUT4(bs-1702R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 63kD

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT4) Polyclonal Antibody, Unconjugated (bs-1702R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Overlay histogram showing HL 60 cells stained with bs-1702R (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (bs-1702R,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-FUT4 (bs-1702R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-FUT4/FITC Conjugated antibody (bs-1702R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.