| 產(chǎn)品編號 | bs-1482R |
| 英文名稱 | CD177 Rabbit pAb |
| 中文名稱 | 嗜中性粒細胞抗原CD177抗體 |
| 別 名 | NB1 GP; PRV-1; HNA-2a; NB1 glycoprotein; Neutrophil Glycoprotein NB1; RGD1562941; CD177 antigen; Polycythemia rubra vera protein 1; PRV-1; NB1 glycoprotein; NB1 GP; Human neutrophil alloantigen 2a; HNA-2a; CD177; NB1; CD177_MOUSE. |
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Specific References (4) | bs-1482R has been referenced in 4 publications.
[IF=3.97] Han, Feifei, et al. "Oral administration of yeast β-glucan ameliorates inflammation and intestinal barrier in dextran sodium sulfate-induced acute colitis." Journal of Functional Foods 35 (2017): 115-126. IHC-P ; Mouse.
[IF=3.73] Scott, Jason A., et al. "The Multifunctional Ca2+/Calmodulin-Dependent Kinase IIdelta (CaMKIIdelta) Regulates Arteriogenesis in a Mouse Model of Flow-Mediated Remodeling." PloS one 8.8 (2013): e71550. IHC-P ; Mouse.
[IF=3.7] Yang, Jian, et al. "Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis." Journal of Cellular and Molecular Medicine (2015). IHC-P ; Rat.
[IF=3.166] Ning YL et al. Caffeine attenuates brain injury but increases mortality induced by high-intensity blast wave exposure. (2019) Toxicology Letters;Feb;301:90-97. IHC ; Mouse.
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| 研究領(lǐng)域 | 免疫學 細胞表面分子 糖蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat CD177: 521-650/818 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014] Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). Similarity: Contains 2 UPAR/Ly6 domains. Gene ID: 499099 Database links: Entrez Gene: 57126 Human Omim: 162860 Human SwissProt: Q8N6Q3 Human Unigene: 232165 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 3: Lymph node (Rat) Lysate at 40 ug
Primary: Anti-CD177 (bs-1482R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90-120 kD
Observed band size: 130 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
