| 產(chǎn)品編號 | bs-1238R |
| 英文名稱 | Rabbit Anti-HO-2 antibody |
| 中文名稱 | 血紅素氧合酶2抗體 |
| 別 名 | Heme oxygenase (decycling) 2; Heme oxygenase (decyclizing) 2; HMOX 2; HMOX2; HMOX2 protein; HO 2; HO2; HMOX2_HUMAN. |
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Specific References (2) | bs-1238R has been referenced in 2 publications.
[IF=6.244] Xinrui Zhang. et al. Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response. Front Oncol. 2021; 11: 696282 WB ; Mouse.
[IF=4.868] Wang Z et al. Aronia melanocarpa Prevents Alcohol-Induced Chronic Liver Injury via Regulation of Nrf2 Signaling in C57BL/6 Mice. Oxid Med Cell Longev. 2020 Jan 8;2020:4054520. WB ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=0.2ug/test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HO-2: 31-150/316 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Summary: catalyzes the conversion of heme to biliverdin; involved in cellular response to oxidative stress [SUBCELLULAR LOCATION] Microsome. Endoplasmic reticulum. [INDUCTION] Heme oxygenase 2 activity is non-inducible. [SIMILARITY] Belongs to the heme oxygenase family. Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Subcellular Location: Microsome. Endoplasmic reticulum. Similarity: Belongs to the heme oxygenase family. Contains 2 HRM (heme regulatory motif) repeats. SWISS: P30519 Gene ID: 3163 Database links: Entrez Gene: 3163 Human Entrez Gene: 15369 Mouse Entrez Gene: 100009523 Rabbit Omim: 141251 Human SwissProt: P30519 Human SwissProt: O70252 Mouse SwissProt: P43242 Rabbit Unigene: 284279 Human Unigene: 272866 Mouse Unigene: 10241 Rat 合成與降解（Synthesis and Degradation） 血紅素氧合酶是目前發(fā)現(xiàn)生物體內(nèi)最易被誘導(dǎo)產(chǎn)生的抗氧化酶類，其抗氧化作用與它的催化分解產(chǎn)物即膽綠素、膽紅素、NO、鐵離子和鐵蛋白有關(guān)。 血紅素氧合酶-2基因缺失對血紅素誘導(dǎo)的氧化應(yīng)激性導(dǎo)致的組織損傷具有保護作用;選擇性抑制神經(jīng)元血紅素氧合酶-2基因的表達可減輕氧化應(yīng)激性組織細胞損傷. |
| 產(chǎn)品圖片 |
Sample:
Brain(Rat)lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-HO-2 (bs-1238R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 38kD
Observed band size : 38kD, 48kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- HO-2 (bs-1238R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 33 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HO-2) Polyclonal Antibody, Unconjugated (bs-1238R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HO-2 Antibody(bs-1238R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
