| 產品編號 | bs-1188R |
| 英文名稱 | FRA2 Rabbit pAb |
| 中文名稱 | fos相關抗原2抗體 |
| 別 名 | FLJ23306; Fos L2; FOS like antigen 2; Fos related antigen 2; FosL 2; FOSL2; FOSL2_HUMAN; FRA 2; fos-related antigen 2. |
| 研究領域 | 腫瘤 信號轉導 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,GuineaPig) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FRA2: 231-326/326 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Fos and Jun dimerize to form Activator Protein 1 (AP1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13 acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the 'leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP1 dependent genes. Function: Controls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts (By similarity). Subunit: Heterodimer Subcellular Location: Nuclear Similarity: Belongs to the bZIP family. Fos subfamily. Contains 1 bZIP (basic-leucine zipper) domain. SWISS: P15408 Gene ID: 2355 Database links: Entrez Gene: 2355 Human Entrez Gene: 421416 Chicken Entrez Gene: 14284 Mouse Omim: 601575 Human SwissProt: P18625 Chicken SwissProt: P15408 Human SwissProt: P47930 Mouse Unigene: 220971 Human Unigene: 596972 Human Unigene: 24684 Mouse Unigene: 10962 Rat Unigene: 163577 Rat FOSL2蛋白對細胞因子、生長因子、感染或致癌刺激等生理或病理信號發(fā)生應答,調節(jié)基因的轉錄, 參與細胞的增殖、分化等過程. FOSL2在腫瘤形成及發(fā)展過程中, 通過促進細胞增殖、抑制分化、促進腫瘤細胞的侵襲和轉移等過程發(fā)揮作用. |
| 產品圖片 |
Sample:
Lane 1: Mouse Small intestine tissue lysates
Lane 2: Rat Small intestine tissue lysates
Primary: Anti-FRA2 (bs-1188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 44 kD
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FRA2/FOSL2 Polyclonal Antibody, Unconjugated(bs-1188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FRA2/FOSL2 Polyclonal Antibody, Unconjugated(bs-1188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-FRA2 antibody (bs-1188R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
