| 產(chǎn)品編號 | bs-1035R |
| 英文名稱 | Rabbit Anti-CD86 antibody |
| 中文名稱 | CD86抗體 |
| 別 名 | CD28LG2; FUN1; LAB72; Activation B7 2 antigen; Activation B7-2 antigen 3; CD86_HUMAN; Activation B7-2 antigen; Activation B72 antigen; B lymphocyte activation antigen B7 2; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7 2 antigen; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD 86; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen; CD86 molecule; CLS1; CTLA 4 counter receptor B7 2; CTLA 4 counter receptor B7.2; CTLA-4 counter-receptor B7.2 2, 3; CTLA4 counter receptor B72; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; TS/A-2. |
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Specific References (63) | bs-1035R has been referenced in 63 publications.
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| 研究領(lǐng)域 | 免疫學(xué) 微生物學(xué) 干細胞 細胞表面分子 糖蛋白 淋巴細胞 t-淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2μg/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 檢測分子量 | 72-74 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of rat CD86: 140-175/313 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]. Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. Subunit: Homodimer. Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed by activated B-lymphocytes and monocytes. Post-translational modifications: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: O35531 Gene ID: 56822 Database links: Entrez Gene: 942 Human Omim: 601020 Human SwissProt: P42081 Human Unigene: 171182 Human Unigene: 229570 Rat CD86是一個很重要要的輔助刺激分子，CD86作為CD28的輔助刺激分子，提供 T 細胞活化的輔助信號,它們之間的結(jié)合和隨后介導(dǎo)的信號轉(zhuǎn)導(dǎo)是 T 細胞和 APC 相互協(xié)作的重要分子基礎(chǔ)。 |
| 產(chǎn)品圖片 |
(UPPER PANEL) 4% Paraformaldehyde-fixed Daudi (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CD86) polyclonal Antibody, unconjugated (bs-1035R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min.
(LOWER PANEL) DAPI (blue, C02-04002) was used to stain the cell nuclei.
The Daudi(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD86 antibody (bs-1035R): 0.5μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
